Human Rap1 modulates TRF2 attraction to telomeric DNA
Our quantitative functional studies explain
how telomeric proteins cooperate in order to
selectively recognize human telomeric DNA.
We investigated human Rap1–TRF2–DNA interactions by quantitative approaches. Our findings could be applied on all proteins that recognize DNA selectively and preserve genome stability.
- Rap1 reduces DNA duplex binding of TRF2
- Rap1 improves TRF2 selectivity for telomeric DNA
by reduction of non-specific electrostatic interactions
- Rap1 induces a partial release of TRF2 from telomeric DNA duplex
We suggested a possible mechanism of how Rap1 affects the recognition of telomeric DNA by TRF2